Paper Chromatography

PAPER CHROMATOGRAPHY Eduardo B. Seletaria Jan Michael Ayuste Jhia Anjela Rivera Dianne Aira Bucad CHEM 2295 BS BIOLOGY 2-1 Group 9 Dennis Macapagal share OF NATURAL SCIENCES COLLEGE OF SCIENCE POLYTECHNIC UNIVERSITY OF THE PHILIPPINES Introduction Paper chromatography is nonpareil system for testing the purity of compounds and identifying substances. Paper chromatography is a recyclable technique because it is relatively quick and requires small quantities of material. Separations in origination word chromatography charter the same principles as those in small point chromatography. In paper chromatography, like thin layer chromatography, substances are distributed among a stationary physique and a restless phase. The stationary phase is usually a piece of highschool tint filter paper. The officious phase is a development etymon that travels up the stationary phase, carrying the samples with it. Components of the sample will discontinue right away according to how strongly they adsorb on the stationary phase versus how readily they dissolve in the mobile phase. METHODOLOGY [Flowchart of steps] (It would be ok if this part would be handwritten) take a leak the developing event and pour 15 ml of the solvent in the developing domiciliate and cover.

Preparation of the Chromatogram by reservation the filter paper rectangular and rank a bulls eye about 1 cm from the edge, 4 tracks are pronounced using the draw and label according to the assigned nutrient color. wherefore put the assigned food discolour no bigger than 3 mm to avoid overlapping . subver t the paper from the chamber and quickly mar! k the location reached by each mark. Then measure te distance travelled by the dye and the distance travelled by the solvent. development Chromatogram: Put at heart the developing chamber the chromatogram when the drifter are dry.The solution should be lower than the spots and reach it undisturbed. Record the results and get the Rf value. Results and Discussion I. solution(Dye) Known Solution RED GREEN no-good YELLOW...If you want to get a full essay, order it on our website: BestEssayCheap.com

If you want to get a full essay, visit our page: cheap essay